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LC/MS/MS Analysis of Steroids on Ascentis® Express C18

LC/MS/MS Analysis of Steroids on Ascentis® Express C18


column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U)
column temp. 40 C
mobile phase [A] 0.1% formic acid in water ; [B] methanol
gradient 5%B to 70%B in 3 minutes, then to 90%B in 0.1 minute, hold 90%B for 0.9 minutes
flow rate 0.5 ml/min
pressure 4351 psi (300 bar)
sample 10 ng/mL in methanol
injection 10 µl
detector MS/MS, ESI(+), MRM (See Figure for transitions)


Analysis Note Fast and selective method for steroids.
Categories Analytical Chromatography, Steroids
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS


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