LC/MS/MS of Pesticides in Oranges on Ascentis® Express C18 after Sample Prep using Supel™ Que Z-Sep/C18 QuEChERS sorbent

LC/MS/MS of Pesticides in Oranges on Ascentis® Express C18 after Sample Prep using Supel™ Que Z-Sep/C18 QuEChERS sorbent

Conditions

sample preparation Dispersive (QuEChERS)
sample/matrix 10 g of pureed oranges (homogenized with rind); spike at 50 ppb (add 16.75 µl of a custom made pesticide mix, each analyte at 30 µg/mL)
extraction tube Supel(TM) QuE Citrate (EN) Tube (55227-U)
extraction process add 10 mL acetonitrile; shake for 1 minute; add contents of a Supel QuE citrate extraction tube (55227-U); shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes
clean-up process transfer 0.7 mL of the acetonitrile layer into a Supel QuE Z-Sep/C18 cleanup tube (55284-U); shake for 1 minute; centrifuge at 5000 rpm for 5 minutes; (transfer 0.2 mL of the supernatant into an empty 1.5 mL centrifuge tube; add 0.2 mL of water; centrifuge at 5000 rpm for 2 minutes.)
clean-up tube Supel-Q(TM) PSA/C18 (55284-U)
column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U)
column temp. 30 C
mobile phase [A] 10 mM ammonium acetate in water; [B] 10 mM ammonium acetate in acetonitrile
gradient Hold at 30% B for 1 min; 30% to 80% B in 2 min; hold at 80% B for 4 min; hold at 100% B for 3 min; hold at 30% B for 3 min
flow rate 0.3 ml/min
pressure 2730 psi (188 bar)
detector MS/MS, ESI positive

Description

Analysis Note Sample pretreatment: 10 g of pureed oranges homogenized with rind; spike at 50 ppb by adding 16.75 μL of a pesticide mix containing each analyte at 30 μg/mL, This demonstrates the application of the Z-Sep/C18 sorbent to the cleanup of fruits in the LC-MS-MS analysis of polar pesticides including organophosphates, carbamates and benzoylureas
Categories Analytical Chromatography, SPE Applications, Pesticides, Pesticides & Herbicides
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC, Supel-Q is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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