LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Gilthead Bream Extract on Ascentis® Express C18 following Extraction by the Folch Method

LC/MS (NARP-LC-APCI(+)-MS) Analysis of Triacylglycerols in Gilthead Bream Extract on Ascentis® Express C18 following Extraction by the Folch Method

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles (53829-U)
mobile phase [A] acetonitrile; [B] 2-propanol (IPA)
gradient 0 to 70% B in 50 min; held at 70% B for 5 min; to 0% B in 1 min
flow rate 1 ml/min
sample fish tissue extracted by Folch method, 1D (Ag+ion) dried fractions dissolved in 200 µl of acetone
injection 5 µl
detector MS, APCI(+), mass range 400-1100 m/z

Description

Analysis Note Data provided by Marco Beccaria (1) and Luigi Mondello (1,2) of (1) Chromaleont s.r.l., c/o "Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, viale Annunziata, 98168 Messina, Italy or (2) #Scienze del Farmaco e Prodotti per la Salute" Department, University of Messina, viale Annunziata, 98168 Messina, Italy.

Triacylglycerols extracted from fish tissue were analyzed by an off-line combination of silver-ion liquid chromatography using a Nucleosil SA column and non-aqueous reversed-phase liquid chromatography (NARP) using an Ascentis Express C18 column. Shown here is how a preliminary separation in the first dimension (Ag+-LC) improves noticeably the quality of the LC analysis of TAG species. Top: TIC chromatogram. From center to bottom: single 1D fractions (from #2 to #6) of the same sample analyzed by NARP-LC-APCI-MS. PN: partition number. DB: double bonds.
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suitability application for LC-MS

Materials

     
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