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LC/MS (TOF) Analysis of 2C Amines Drugs of Abuse in Urine on Ascentis® Express HILIC after Solid Phase Extraction (SPE) using Supel™-Select SCX

LC/MS (TOF) Analysis of 2C Amines Drugs of Abuse in Urine on Ascentis® Express HILIC after Solid Phase Extraction (SPE) using Supel™-Select SCX

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix spiked urine, 1 mL
SPE well plate Supel-Select SCX SPE 96-well Plate, 30 mg/well (575664-U)
condition 1 mL methanol, then 1 mL 0.1% formic acid in water
washing 2 x 1 mL 10% methanol in water, 2 x 1 mL 1% formic acid in methanol
elution 2 mL 5% ammonium hydroxide in acetonitrile
eluate post-treatment evaporate to 500 µl with nitrogen at room temperature, reconstitute in 250 µl acetonitrile
column Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm particles (53939-U)
column temp. 35 C
mobile phase 5 mM ammonium formate in acetonitrile:water (90:10, v/v)
flow rate 0.4 ml/min
pressure 1570 psi (108 bar)
injection 2 µl
detector TOF/MS

Description

Analysis Note The 2C amines are a family of illicit, psychedelic phenethylamines. The term "2C" refers to the two carbon atoms between the benzene ring and the amino group in the parent molecular structure. A selection of 2C amines is shown here extracted from urine using Supel-Select SCX polymeric SPE, separated on an Ascentis Express HILIC column, and detected by TOF-MS. Cerilliant CRMs were used to ensure reliable MS identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, SPE Applications, Drugs of Abuse, drugs of abuse, Drugs of abuse, Pharmaceutical & Clinical applications
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for SPE

Materials

     
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