LC/MS (TOF) Analysis of Dermorphin on Ascentis® Express F5

LC/MS (TOF) Analysis of Dermorphin on Ascentis® Express F5

Conditions

column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53569-U)
column temp. 35 C
mobile phase 2 mM ammonium formate (90:10 acetonitrile:water) adjusted to pH 4.5 with formic acid
flow rate 0.4 ml/min
sample 10 µg/mL in 75:25 acetonitrile:water
injection 1 µl
detector ESI(+), TIC 100 - 1000 m/z; APCI (+), XIC m/z = 150.1, 203.7, 378.2

Description

Analysis Note Dermorphin is a heptapeptide derived from frogs. It is a painkiller and used illicitly in racehorses so they continue to run through the pain. A rapid analysis of dermorphin standard is shown here on an Ascentis Express F5 column. The HILIC mode separation provided the necessary retention and MS-compatibility, while the pentafluorophenyl (F5) phase chemistry elimated the chelating interactions that are problematic on bare silica.
Categories Analytical Chromatography, Forensics and Toxicology, SPE Applications, peptides, drugs of abuse, Peptides, Drugs of abuse, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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