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LC/MS (TOF) Analysis of Petunidin Glycosides from High Bush Blueberries on Ascentis® Express C18

LC/MS (TOF) Analysis of Petunidin Glycosides from High Bush Blueberries on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 35 C
mobile phase [A] 0.1% (v/v) formic acid in water; [B] 0.1% (v/v) formic acid in 75:25 (v/v) acetonitrile:water
gradient 2% B for 2 min, to 100% B in 38 min
flow rate 0.2 ml/min
detector ESI(+) TOF, extracted ions m/z 479.1192, 449.1086, 521.1323

Description

Analysis Note The purpose of this study was to evaluate the change in anthocyanin levels in wild blueberries as a function of berry development. The delphinidin, putinidin, peonidin, and malvinidin base-modified components exhibited a dramatic increase in abundance as a function of berry development. The cyanidin base-modified components  exhibited a much lower increase in abundance as a function of berry development, it is uncertain the significance of the rate increase. Chlorogenic acid exhibited a continuous decrease in abundance as a function of berry development. Overall, the combination of UV/Vis detection along with accurate mass TOF enabled a effective method of identification of anthocyanins from blueberry extracts. The Ascentis Express column provided rapid, sensitive detection and rugged operation.
Categories Analytical Chromatography, Antioxidants
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Berries (1.0 g) were added to 1.0 mL of 1% (v/v) formic acid in methanol. Samples were crushed in the solvent mixture and extracted (refrigerated) for 2 hours. A portion of the extracted sample was removed, centrifuged and the supernatant was collected for HPLC analysis.
suitability application for LC-MS

Materials

     
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