Lot-to-Lot Reproducibility of Monoclonal Antibody Analysis on Three Lots of Zenix® SEC-300 Columns

Lot-to-Lot Reproducibility of Monoclonal Antibody Analysis on Three Lots of Zenix® SEC-300 Columns

Conditions

column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm, 300 Ã… (Z777033)
column temp. Ambient
mobile phase 150 mM Phosphate Buffer, pH 7
flow rate 1.0 ml/min
sample 2.5 mg/ml MAb
injection 10 µl
detector UV, 214 nm

Description

Analysis Note Exceptional lot-to-lot reproducibility is demonstrated for three lots of 3 micron Zenix SEC-300 columns for the release test of monoclonal antibody-based therapeutics. Monomer retention time, peak efficiency and peak height are shown to be virtually identical on the three lots.
Categories Analytical Chromatography, Antibodies
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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