Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins

Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins

Conditions

column TSKgel Phenyl-5PW, 7.5 cm x 7.5 mm, 20 µm particles (843277)
column TOYOPEARL Phenyl-650M, 7.5 cm x 7.5 mm, 65 µm particles (819818)
column TSKgel Phenyl-5PW, 7.5 cm x 7.5 mm, 30 µm particles (843177)
gradient for the 10 and 30 µm TSKgel columns: 0 to 100% B in 60 min;
gradient for the 65 µm TOYOPEARL columns: 0 to 100% B in 240 min
flow rate for the 10 and 30 µm TSKgel columns: 68 cm/hr;
flow rate for the 10 and 30 µm TSKgel columns: 68 cm/hr;for the 65 µm TOYOPEARL columns: 272 cm/hr
injection 100 muL containing 1 mg/mL each protein
detector UV, 280 nm

Description

Analysis Note TSKgel and TOYOPEARL particles were packed into 7.5 cm x 7.5 mm columns for this comparison. The part numbers referenced are for the bulk packing. Empty glass columns are sold separately.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation, Toyopearl is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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