UHPLC Analysis of Antihistamines on Ascentis® Express C18

UHPLC Analysis of Antihistamines on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 5 µm particles (50526-U)
column temp. 40 °C
mobile phase [A] 0.02M sodium phosphate, pH 2.6; [B] methanol
gradient 50% B for 0.5 min; to 75% B in 2 min; held for 2 minutes
flow rate 1.0 mL/min
pressure 2770 psi (191 bar)
sample Dissolved in 20:80 (v:V0 water:methanol
injection 1.0 µL
detector UV, 230 nm

Description

Categories Analytical Chromatography, Antihistamines
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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