UHPLC Analysis of Diazepam and Metabolites on Titan™ C18, Competitive Comparison

UHPLC Analysis of Diazepam and Metabolites on Titan™ C18, Competitive Comparison

Conditions

column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 °C
mobile phase [A] 0.1% formic acid; [B] acetonitrile; (60:40, A:B)
pressure 6450 psi (445 bar)
detector UV, 254 nm

Description

Analysis Note This application shows a comparison between Titan C18 and a competitive column for diazepam and its metabolites using a MS compatible mobile phase.
Categories Analytical Chromatography, Clinical, Forensics and Toxicology, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure, Drugs of Abuse, antiepileptic/antiseizure, antidepressants, Antidepressants, drugs of abuse
Featured Industry Clinical
Forensics and Toxicology
Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
Other Notes Used 75 μm I.D. x 250 mm L tubing in system.
suitability application for UHPLC

Materials

     
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