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UHPLC Analysis of Fat Soluble Vitamins on Titan™ C18, Methanol Mobile Phase

UHPLC Analysis of Fat Soluble Vitamins on Titan™ C18, Methanol Mobile Phase

Conditions

column Titan C18, 50 mm x 2.1 mm I.D., 1.9 µm particles (577122-U)
column temp. 30 C
mobile phase 100% methanol
flow rate 0.4 ml/min
pressure 2741 psi (189 bar)
sample All compounds at 0.5 mg/mL except retinyl acetate at 0.05 mg/mL in methanol
injection 0.5 µl
injection UV, 280 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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