UHPLC Analysis of Horny Goat Weed Standards using Titan™ C18

UHPLC Analysis of Horny Goat Weed Standards using Titan™ C18

Conditions

column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 °C
mobile phase [A] 0.1% acetic acid in water; [B] 0.1% acetic acid in acetonitrile;
gradient 25% B held for 3 min; to 100% B in 6 min; 100% B held for 2 min
flow rate 0.4 mL/min
pressure 5130 psi (354 bar)
sample 50 µg/mL 70:30, water:methanol
injection 5 µL
detector UV, 250 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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