UHPLC Analysis of Iloperidone and Its Primary Metabolite Hydroxyiloperidone on Ascentis® Express C18

UHPLC Analysis of Iloperidone and Its Primary Metabolite Hydroxyiloperidone on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 3.0 mm, I.D., 2.0 µm particles (50819-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate in water, pH adjusted to 3.0 with formic acid [B] 0.1% formic acid in methanol
gradient 45% B for 2 min; to 80% B over 10 min; held at 80% B for 2 min
flow rate 0.5 mL/min
pressure 6500 psi (445 bar)
sample 50 µg/mL Iliperidone, 5 µg/mL hydroxyiliperidone in 10% methanol
injection 2 µL
detector UV, 275 nm

Description

Analysis Note Iloperidone is an atypical antipsychotic administered for the treatment of schizophrenia. The drug is marketed under the trade names Fanapt and Fanapta. Hydroxyiloperidone is a major metabolite of iloperidone. The metabolite is also pharmacologically active, possessing central nervous system (CNS) activity. Shown here is the rapid, efficient UHPLC separation of iloperidone and hydroxyiloperidone on a Fused-Core Ascentis Express C18 column. Cerilliant CRMs provided reliable quantification.
Categories Analytical Chromatography, Antipsychotics
Featured Industry Clinical
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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