UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18

UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 µm particles (50813-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (90:10, A:B)
flow rate 0.7 mL/min
pressure 1153 psi (795 bar)
sample 50 µg/mL in 85:15, water:methanol
injection 1 µL
detector UV, 254 nm

Description

Analysis Note Methotrexate (MTX) has been selected as a tracer of surface contamination exposure of hospital staff to antineoplastic drugs (ANDs). Its analysis is an emerging facet of occupational medicine in hospital premises. Methotrexate and the related compounds aminopterin and folic acid are shown here separated by UHPLC on an Ascentis Express C18 column in less than two minutes. The rapid, high resolution separation means the method can be employed in hospitals or clinical settings to routinely monitor the contamination levels of surfaces.
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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