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UHPLC Analysis of Piroxicam and Impurity (2-Aminopyridine) on Ascentis® Express F5

UHPLC Analysis of Piroxicam and Impurity (2-Aminopyridine) on Ascentis® Express F5

Conditions

column Ascentis Express 10 cm x 2.1 mm I.D., 2.0 µm particles (50863-u)
column temp. 35 C
mobile phase [A] 10 mM ammonium formate pH 3.0 with formic acid; [B] acetonitrile; (75:25, A:B)
flow rate 0.5 ml/min
pressure 7570 psi (522 bar)
sample 5 - 100 µg/mL in 90:10, water:methanol
injection 0.5 µl
detector UV, 250 nm

Description

Analysis Note Piroxicam, an NSAID (non-steroidal antiinflammatory drug), and a common piroxicam impurity, 2-aminopyridine, are shown here separated by UHPLC on an Ascentis Express F5 column. The unique phase chemistry of the F5 (pentafluorophenyl) stationary phase has the selectivity to resolve these closely related compounds.
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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