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UHPLC Analysis of PP242 (2-(4-Amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol) on Ascentis® Express RP-Amide

UHPLC Analysis of PP242 (2-(4-Amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol) on Ascentis® Express RP-Amide


column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (80:20, A:B)
flow rate 0.4 ml/min
pressure 3050 psi (210 bar)
injection 1 µl
detector UV, 254 nm


Analysis Note PP242 is a potent and selective mTOR inhibitor and is a first selective inhibitor that targets ATP domain of mTOR. From a method development standpoint, PP243 is an interesting compound as it has several hydrogen bond donor groups. For this reason, the Ascentis® Express RPA-Amide phase is a good starting point for the separation.
Categories Analytical Chromatography, Drugs, miscellaneous
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Stock solution prepared in DMSO, 1 mg/mL
suitability application for UHPLC


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