UHPLC Analysis of Stilbenes on Ascentis® Express C8 and ES-Cyano phases

UHPLC Analysis of Stilbenes on Ascentis® Express C8 and ES-Cyano phases

Conditions

column Ascentis Express ES-Cyano, 5 cm x 4.6 mm I.D., 5 µm particles (50581-U)
column Ascentis Express C8, 5 cm x 4.6 mm I.D., 5 µm particles (50389-U)
column temp. 30 C
mobile phase [A] water; [B] acetonitrile
gradient 40-60% B in 3 minutes; hold at 60% B until 4 minutes
flow rate 2.0 ml/min
pressure 1740 psi (120 bar)
sample Dissolved in 50/50 water/ACN
injection 1 µl
detector UV, 230 nm

Description

Categories Analytical Chromatography, Antioxidants
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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