UHPLC Analysis of Sulfa Drugs on Ascentis® Express C18

UHPLC Analysis of Sulfa Drugs on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 µm particles (50813-U)
column temp. 45 °C
mobile phase [A] 0.1% acetic acid; [B] 0.1% acetic acid in acetonitrile; (90:10, A:B)
flow rate 0.8 mL/min
pressure 10720 psi (739 bar)
sample 25 µg/mL in 90:10, water:methanol
injection 0.5 µL
detector UV, 250 nm

Description

Analysis Note The UHPLC method presented here shows the rapid analysis of the sulfa drugs sulfacetamide, sulfadiazine, sulfathiazole, and sulfamerazine on Ascentis Express 2 μm C18. Analysis time is less than one minute. The sulfa drugs analyzed here all contain a sulfanilamide substructure as active group.
Categories Analytical Chromatography, Drugs, miscellaneous
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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