UHPLC Analysis of Tretinoin and Related Compounds on Ascentis® Express C18

UHPLC Analysis of Tretinoin and Related Compounds on Ascentis® Express C18

Conditions

column Ascentis Express C18 5 cm x 2.1 mm, 2.0 µm particle size (50811-U)
column temp. 50 °C
mobile phase (A) water with 5% acetic acid; (B) methanol with 5% acetic acid; (70:30, A:B)
flow rate 0.5 mL/min
pressure 5240 psi (361 bar)
sample Tretinoin and related 20-50 µg/mL in mobile phase
injection 0.7 µL

Description

Categories Analytical Chromatography, Pharmaceuticals
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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