UHPLC Analysis of Vitamin K1 and Related Compound on Ascentis® Express C18

UHPLC Analysis of Vitamin K1 and Related Compound on Ascentis® Express C18

Conditions

column Ascentis Express C18 15 cm x 4.6 mm I.D., 5 µm particles (50537-U)
column temp. 35 °C
mobile phase [A] methanol; [B] 2-propanol; (90:10, A:B)
flow rate 1.0 mL/min
pressure 1035 psi (71 bar)
sample Vitamin K1 and related, 50 µg/mL 90:10, methanol:2-propanol
injection 2 µL
detector UV, 250 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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