UHPLC Analysis of Aldehyde and Ketone DNPH Derivatives on Ascentis® Express C18

UHPLC Analysis of Aldehyde and Ketone DNPH Derivatives on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 µm particles (50813-U)
column temp. 45 °C
mobile phase [A] water; [B] acetonitrile; (40:60, A:B)
flow rate 0.8 mL/min
pressure 9520 psi (656 bar)
sample CARB Method 1004 DNPH Mix 2 (47651-U) diluted 10:1 in 40:60, water:acetonitrile
injection 0.1 µL
detector UV, 360 nm

Description

Analysis Note Environmental contaminants analyzed by this UHPLC separation are a standard mix of aldehydes and kethones as their DNPH derivatives. Formaldehyde is the target compound for US EPA Compendium Method TO-11A and Method 8315, but at least twelve other carbonyl compounds also can be detected and quantified.
Categories Analytical Chromatography, Air Monitoring Applications, Aldehydes & Ketones
Featured Industry Environmental
Industrial Hygiene
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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