UHPLC Gradient Analysis of Boswellia Herbal Supplement on Titan™ C18

UHPLC Gradient Analysis of Boswellia Herbal Supplement on Titan™ C18

Conditions

extraction 1. Weigh 100 mg Bosewellia Herbal Supplement     2. Add 10 mL ethanol     3. Sonicate 30 min at 40C     4. Dilute 100 µL extract, 400 µL ethanol, 500 µL water
column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 °C
mobile phase [A] 0.1% phosphoric acid; [B]acetonitrile
gradient 40% B for 0.5 min; to 100% B in 2 min; held at 100% B for 10 min
flow rate 0.4 mL/min
pressure 4050 psi (279 bar)
sample Bosewellia Extract in 50:50, water:ethanol
injection 2 µL
detector UV, 210 nm

Description

Categories Analytical Chromatography, Herbal medicines
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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