UHPLC Gradient Analysis of Boswellic Acids on Ascentis® Express Phenyl-Hexyl

UHPLC Gradient Analysis of Boswellic Acids on Ascentis® Express Phenyl-Hexyl

Conditions

column Ascentis Express Phenyl-Hexyl, 10 cm x 3.0 mm I.D., 2.7 µm particles (53345-U)
column temp. 35 °C
mobile phase [A] 0.1% phosphoric acid; [B] acetonitrile
gradient 50% B for 0.5 min; to 100% B in 4.5 min; held at 100% B for 5 min
flow rate 0.6 mL/min
pressure 2390 psi (165 bar)
sample 6 Boswellic Acids 50µg/mL in 70:30, water:methanol
injection 2 µL
detector UV, 210 nm

Description

Categories Analytical Chromatography, Herbal medicines
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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