UHPLC/MS Analysis of 8-Hydroxy-2′-Deoxyguanosine and Related Compounds on Titan™ Silica

UHPLC/MS Analysis of 8-Hydroxy-2′-Deoxyguanosine and Related Compounds on Titan™ Silica

Conditions

column Titan Silica, 10 cm x 3.0 mm I.D., 1.9 µm particles (581536-U)
column temp. 35 C
mobile phase [A] acetonitrile; [B] 100 mM ammonium formate, pH 3.0 with formic acid; (90:10, A:B)
flow rate 0.6 ml/min
pressure 2060 psi (142 bar)
sample 1 µg/mL in 99:1, acetonitrile:water
injection 2 µl
detector MS ESI+, combined SIR, 268, 284 m/z

Description

Analysis Note The rapid, efficient separation of these highly polar compounds on a Titan Silica column in HILIC mode is shown here.
Categories Analytical Chromatography, Nucleosides
Featured Industry Life Science and Biopharma
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC-MS

Materials

     
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