UHPLC-MS Analysis of Basic Drugs and Metabolites with Titan™ C18

UHPLC-MS Analysis of Basic Drugs and Metabolites with Titan™ C18

Conditions

column Titan C18 10 cm x 2.1 mm, 1.9 µm particles (577124-U)
column temp. 25 C
mobile phase [A] Water; [B] acetonitrile, both with 0.1% formic acid
gradient 35%B Isocratic
flow rate 0.4 ml/min
pressure 484 psi (7120)
sample 50 ng/mL in 50% methanol
injection 1 µl
detector MS, ESI(+), MRM

Description

Categories Analytical Chromatography, Pharmaceuticals
Featured Industry Life Science and Biopharma
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
Other Notes MRM transitions
1. Quinidine 325.2/81.2
2. Propranolol 260.1/116.2
3. Haloperidol 376.1/165.1
4. Haloperidol metabolite II 378.1/149.1
5. Haloperidol cholor analog 392.1/181.0
6. Fluoxetine 310.1/44.2
suitability application for UHPLC-MS

Materials

     
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