UHPLC/MS Analysis of Estradiols using Ascentis® Express C18

UHPLC/MS Analysis of Estradiols using Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1mm, 2 µm particles (50813-U)
column temp. 30 C
mobile phase [A] water; [B] methanol
gradient 30 to 40% B in 0.1 min; 40 to 60% B in 0.9 min; 60 to 75% B in 6 min; 75 to 30% B in 0.1 min; held at 30% B for 2.9 min
flow rate 0.2 ml/min
pressure 6544 psi (451 bar)
sample in 20% methanol -80% water at 10 ng/mL
injection 10 µl
detector MS, ESI (-), 271/145

Description

Analysis Note Standard at 10 ng/mL.
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

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