UHPLC/MS Analysis of Flakka (α-PVP) and α-Pyrrolidinobutiophenone in Synthetic Urine on Titan™ Silica 1.9 μm

UHPLC/MS Analysis of Flakka (α-PVP) and α-Pyrrolidinobutiophenone in Synthetic Urine on Titan™ Silica 1.9 μm

Conditions

sample preparation 100 µl of synthetic urine spiked with 200 ng/mL of each analyte, diluted to 1 mL with acetonitrile, vortex 30 sec,   (centrifuge at 10,000 rpm for 1 min, filter using 0.45 µm Millex syringe filter prior to UHPLC analysis)
column Titan Silica, 10 cm x 3.0 mm I.D., 1.9 µm particles (581536-U)
column temp. 35 C
mobile phase 10 mM ammonium formate in 95:5, acetonitrile:water
flow rate 0.8 ml/min
pressure 2585 psi (178 bar)
sample urine spiked at 200 ng/mL each analyte
injection 2 µl
detector MS ESI+, combined SIR, 218, 232 m/z

Description

Analysis Note The simple extraction from urine and rapid separation of the street drug Flakka (α-PVP) and a related compound is shown here on a Titan Silica column in HILIC mode. Highest grade UHPLC solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC-MS

Materials

     
Related Links