UHPLC/MS Analysis of Flakka (α-PVP) and α-Pyrrolidinobutiophenone in Synthetic Urine on Ascentis® Express HILIC 2.7 μm

UHPLC/MS Analysis of Flakka (α-PVP) and α-Pyrrolidinobutiophenone in Synthetic Urine on Ascentis® Express HILIC 2.7 μm

Conditions

column Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 µm particles (53934-U)
column temp. 35 Cambient
mobile phase 5 mM ammonium formate in 95:5, acetonitrile:water
flow rate 0.5 ml/min
pressure 3275 psi (226 bar)
sample as prepared
injection 2 µl
detector MS ESI+, MRM, 218.12 > 90.98, 232.13 > 91.04 m/z

Description

Analysis Note The simple extraction from urine and rapid separation of the street drug Flakka (α-PVP) and a related compound is shown here on an Ascentis Express HILIC column in HILIC mode. Highest grade UHPLC solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes matrix: 100 μL of horse plasma spiked with 200 ng/mL of each analytediluant:  900 μL acetonitrilevortex: 30 seccentrifuge:  10,000 rpm for 1 minfilter:  0.45 μm (Z227544)
suitability application for UHPLC

Materials

     
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