UHPLC/MS Analysis of Morphine and Metabolites on Titan™ C18

UHPLC/MS Analysis of Morphine and Metabolites on Titan™ C18

Conditions

column Titan C18, 5 cm x 2.1 mm I.D., 1.9 µm particles (577122-U)
column temp. 25 °C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid ; (95:5, A:B)
flow rate 0.4 mL/min
pressure 4960 psi (342 bar)
sample 1 µg/mL in 99:1, water:methanol
injection 0.5 µL
detector MS-TOF, XIC

Description

Analysis Note This method shows morphine and its metabolites separated on a Titan C18 column and MS detection. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, Drugs of Abuse, drugs of abuse
Featured Industry Clinical
Forensics and Toxicology
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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