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UHPLC/MS Analysis of Spice Cannabinoids on Titan™ C18

UHPLC/MS Analysis of Spice Cannabinoids on Titan™ C18

Conditions

column Titan C18, 10 cm x 2.1 mm, 1.9 µm particles (577124-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in 95:5, water:acetonitrile; [B] 0.1% formic acid in 5:95, water:acetonitrile;
gradient 25% B held for 1 min; to 45% B in 0.5 min; 45% B held for 4.5 min;   to 80% B in 0.5 min; 80% B held for 4 min
flow rate 0.5 ml/min
pressure 8756 psi (604 bar)
sample 100 ng/mL in 60:40, water:methanol
injection 2 µl
detector MS-ESI+,100 - 1000 m/z scan, XIC

Description

Analysis Note Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provides a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. The rapid, efficient UHPLC separation a set of these compounds on a Titan C18 column is shown here. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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