UHPLC/MS Analysis of Vitamin K1 and Related Compound on Ascentis® Express C18

UHPLC/MS Analysis of Vitamin K1 and Related Compound on Ascentis® Express C18

Conditions

column Ascentis Express C18 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U)
column temp. 35 °C
mobile phase [A] methanol; [B] 2-propanol; (90:10, A:B)
flow rate 0.4 mL/min
pressure 1870 psi (129 bar)
sample 50 ng/mL 90:10, methanol:2-propanol
injection 2 µL
detector MS-APCI+, full scan 425-525 m/z

Description

Categories Analytical Chromatography, Clinical, vitamins, Vitamins and Natural products
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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