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Higher Efficiency of Ascentis® Express 2.7 μm Compared to 3 μm Particles Gives Better Sensitivity

Higher Efficiency of Ascentis® Express 2.7 μm Compared to 3 μm Particles Gives Better Sensitivity

Conditions

column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles and C18, 5 cm x 2.1 mm I.D., 3 µm particles (53822-U)
column temp. 35 C
mobile phase [A] 25 mM dibasic ammonium phosphate (pH 7.0): [B] water: [C] acetonitrile; (35:0:65 or 35:4:61, A:B:C)
flow rate 0.2 ml/min
injection 1 µl
detector UV, 220 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Antiarrythmic, Antidepressants, Antihistamines
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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