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USP Analysis of Actos (Pioglitazone) using Ascentis® Express C18

USP Analysis of Actos (Pioglitazone) using Ascentis® Express C18


column Ascentis(R) Express C18, 15 cm x 4.6 mm, 5 µm particles (50537-U)
column temp. 25 C
mobile phase [A] 100 mM ammonium acetate; [B] acetonitrile; [C] acetic acid; (55:45:2, A:B:C)
flow rate 0.7 ml/min
pressure 1160 psi (80 bar)
sample pioglitazone 50 µg/mL, benzophenone 13 µg/mL in mobile phase
injection 20 µl
detector UV, 269 nm


Categories Analytical Chromatography, Antihyperglycemic
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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