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Analytical Method: Calcium in Beer

Instruments and Reagents:

Cat.No. 173016  Spectroquant® VIS Spectrophotometer Prove 100 or

Cat.No. 173017  Spectroquant® UV/VIS Spectrophotometer Prove 300 or

Cat.No. 173018  Spectroquant® UV/VIS Spectrophotometer Prove 600 or

Cat.No. 114946  Rectangular cells 10 mm and/or

Cat.No. 114947  Rectangular cells 20 mm and/or

Cat.No. 114944  Rectangular cells 50 mm
Cat.No. 100049  Spectroquant® Calcium Test
Cat.No. 116754  Water for analysis
Cat.No. 114946  Spectroquant® Rectangular cells 10 mm

   
Sample preparation:
Fill 100 ml of beer in a 600 ml beaker. Stir in order to drive off the CO2 for about 1 hour with a magnetic stirrer. Alternatively you can use also an ultra sonic bath. Attention! Foams strongly.
 
Dilution steps:
Samples containing higher than 4.00 mg/l Ca must be diluted with water for analysis according to the following table:

   

Expected original beer concentration

volume of sample

Filling up in a volumetric flask with x ml volume

Dilution factor

25 mg/l

8 ml

100

12.5

50 mg/l

4 ml

100

25

100 mg/l

2 ml

100

50

200 mg/l

1 ml

100

100

 

 

 

 

 

 

 

 

 

 


Therefore pipette x ml of this solution into a 100-ml volumetric flask and fill up to volume with water for analysis to the mark (= diluted beer sample).
 
Analysis:

Determine the CA content with the above mentioned test kit against a reagent blank (water for analysis (instead of beer) + reagents).

The method is preprogrammed on the Prove instruments, but has to be calibrated for each batch.
For details see Prove manual.
It is recommended to perform a calibration with 5 - 10 measurement points over the entire measuring range.
The calibration should be checked regulary using standard solutions.

 

Materials

     
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