Analytical Method: Iron (total) in wine

Instruments and Reagents:

Cat.No. 173016  Spectroquant® VIS Spectrophotometer Prove 100 or

Cat.No. 173017  Spectroquant® UV/VIS Spectrophotometer Prove 300 or

Cat.No. 173018  Spectroquant® UV/VIS Spectrophotometer Prove 600 or

Cat.No. 109748  Spectroquant® Photometer NOVA 30 or

Cat.No. 109751  Spectroquant® Photometer NOVA 60 or

Cat.No. 173632  Spectroquant® Colorimeter Move 100

Cat.No. 114946  Rectangular cells 10 mm and/or

Cat.No. 114947  Rectangular cells 20 mm and/or

Cat.No. 114944  Rectangular cells 50 mm
Cat.No. 114549  Spectroquant® Iron Cell Test
Cat.No. 114761  Spectroquant® Iron Test
Cat.No. 100796  Spectroquant® Iron Test
Cat.No. 100731  Sulfuric acid 95 - 97 % for analysis
Cat.No. 107210  Hydrogen peroxide 30 % (Perhydrol®) for analysis
Cat.No. 105590  Sodium hydroxide solution 32 %
Cat.No. 109531  MColorpHastTM  pH-indicator strips pH 0 - 6
Cat.No. 116754  Water for analysis
 
Sample preparation:
Samples of wine have to be treated with acid for determination. The various components of wine can cause an under-valuation, if no sample preparation is done.
In a 100-ml Erlenmeyer flask evaporate 10 ml of the sample to about 1 ml on the heating plate at low heat. Mix the residue with 2 ml sulfuric acid 95 - 97 %. After cooling add 1 ml Perhydrol®, mix and heat again on the heating plate. After the reaction (foaming) has faded, once more add 1 ml Perhydrol® and heat under addition of a glass pearl, until a white SO3 smoke arises. If the sample turns brown, again add 1 ml Perhydrol® after cooling and heat till the SO3 smoke arises. Afterwards the residue should be clear and colorless to light yellow.
After cooling dissolve the residue in 5 ml water for analysis and adjust to pH 3 - 4 with 5.5 - 6 ml sodium hydroxide solution 32 % (pH-indicator strips). Transfer the solution into a 25-ml volumetric flask, fill up to the mark with water for analysis and mix.
 
Analysis:
Determine with the above mentioned test kits.
 
Calculation:
Iron (total) content in mg/l Fe = analysis value in mg/l Fe x 2.5

Materials

     
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