Analytical Method: Nitrite in meat and sausage products

Photometric determination using the Griess method subsequent to extraction with water

Instruments and Reagents:

Cat.No. 173016  Spectroquant® VIS Spectrophotometer Prove 100 or

Cat.No. 173017  Spectroquant® UV/VIS Spectrophotometer Prove 300 or

Cat.No. 173018  Spectroquant® UV/VIS Spectrophotometer Prove 600 or

Cat.No. 109748  Spectroquant® Photometer NOVA 30 or

Cat.No. 109751  Spectroquant® Photometer NOVA 60 or

Cat.No. 173632  Spectroquant® Colorimeter Move 100

Cat.No. 114946  Rectangular cells 10 mm and/or

Cat.No. 114947  Rectangular cells 20 mm and/or

Cat.No. 114944  Rectangular cells 50 mm
Cat.No. 114547  Spectroquant® Nitrite Cell Test
Cat.No. 114776  Spectroquant® Nitrite Test
Cat.No. 110537  Carrez Clarification
Cat.No. 109137  Sodium hydroxide solution 1 mol/l
Cat.No. 116754  Water for analysis
 
Sample preparation:
In an Erlenmeyer-flask weigh exactly 10 g of the sample and mix with about 80 ml water for analysis. With the Ultra-Turrax high speed blender homogenize the mixture for 60 seconds. With 50 ml hot water for analysis rinse the shaft of the homogenizer into the flask, adding it to the mixture. Then adjust the pH-value of the solution to
7 - 7.2 with sodium hydroxide solution 1 mol/l, using the pH-meter, and heat for 15 minutes in a bath of boiling water, while occasionally shaking. After cooling down to room temperature quantitatively transfer the prepared sample into a 200-ml standard volumetric flask and successively mix it with 2 ml Carrez solution-1 and -2 at a time. With rich in connective tissue products use 4 ml Carrez solution-1 and -2 respectively. Shake after each addition. Then fill up to volume with water for analysis and, after mixing, filter through a folded filter. Discard the first filtrate and use the remaining, clear filtrate for the determination.
 
Analysis:
Determine with the above mentioned test kits.
 
Calculation:
Nitrite content in mg/kg NO2 = analysis value in mg/l NO2 x 20
Nitrite content in mg/kg NaNO2 = analysis value in mg/l NO2 x 30

Materials

     
Related Links

Categories