TLC Analysis of Cholesterol and its Bile Acid Metabolites on HPTLC Pre-Coated Plate RP-18 F254s

TLC Analysis of Cholesterol and its Bile Acid Metabolites on HPTLC Pre-Coated Plate RP-18 F254s

Conditions

sample 0.4 µL
detector dipped in dichlorofluorescein (0.2% in ethanol) (Spray reagent: MnCl2 - sulfuric acid
heated up to 120 °C for 120 min, UV 366 nm)
plate TLC pre-coated plate RP-18 F 254s (1.15389.0001,1.15423.0001)
eluent [A] methanol; [B] acetone; [C] IN acetic acid; (85:10:5)
migration distance 10 cm
chamber normal chamber without chamber saturation

Description

Categories Analytical Chromatography, TLC Applications, sterols
Featured Industry Life Science and Biopharma
General description TLC Plate Information: 1.15389.0001
suitability application for TLC

Materials

     
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