TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 wF254s

TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 wF254s

Conditions

sample 5 µL
chamber normal chamber without chamber saturation
eluent [A] methanol; [B] acetonitrile; [C] water; (30:70:5, A:B:C)
plate HPTLC pre-coated plate RP-18 wF 254s with concentrating zone (1.13124.0001)
detector
Dipped in MnCl2-sulfuric acid heated up to 110 °C for 10 min in situ evaluation
(with TLC/HPTLC scanner (Camag) UV 366 nm)
migration distance 6 cm

Description

Featured Industry Life Science and Biopharma
suitability application for TLC

Materials

     
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