Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in Plasma

By: Craig Aurand, Reporter US Volume 31.1

For efficient therapeutic drug monitoring, it is important for clinicians to have access to fast and robust analytical methods to ensure accurate assessment of drug efficacy. Industrial trends toward highly specific LC/MS applications over traditional immunoassay have resulted in the need for high-speed chromatographic assays along with simplified sample preparation methods. Often the limitation of a bioanalytical technique is based upon the effectiveness of the sample preparation technique. Plasma and serum samples are often susceptible to assay irregularities due to matrix-induced interferences. In this study the impact of matrix interference is investigated with respect to precision and accuracy of antiarrhythmic cardiac drugs from plasma samples.

Therapeutic Drug Monitoring of Antiarrhythmic Drugs

The focus of the study reported here was to develop a bioanalytical assay for antiarrhythmic cardiac drugs and associated metabolites from plasma samples. The goal was to develop a robust analytical technique that facilitates high throughput application with simplified sample processing. Figure 1 shows the structures of the antiarrhythmic drugs evaluated in this study. The utility of these antiarrhythmic drugs vary; lidocaine is used for ventricular arrhythmias, flecainide is administered for tachyarrhythmia, and amiodarone is utilized in both ventricular and atrial cardiac dysrhythmias1. Having an analytical method capable of analyzing a range of antiarrhythmic agents allows for simplified analysis while increasing the services the testing laboratory can offer. This not only pertains to LC/MS analysis, but also to sample collection and sample preparation.

Structures of Antiarrhythmic Compounds

Figure 1. Structures of Antiarrhythmic Compounds

Analytical Approach

The first part of the study focused on the chromatographic conditions for resolution of lidocaine, flecainide, amiodarone and the associated metabolite, desethylamiodarone. The basic nature of these antiarrhythmic agents makes them ideal candidates for HILIC (hydrophilic interaction) chromatographic separation. The benefit of HILIC over traditional reversed-phase chromatography is twofold, for both sample introduction and analyte detection2. First, the high acetonitrile concentration of HILIC mobile phases allows for direct analysis of precipitated plasma samples without the need for additional sample solvent exchange. Second, the high acetonitrile content provides increased analyte response in positive ESI MS detection. Of the various HILIC-mode columns tested, method development for this assay determined the Ascentis® Express HILIC, 2.7 µm particles, provided the best chromatographic resolution of the antiarrhythmic drugs while maintaining high peak efficiency for enhanced detection levels.

Once the chromatographic and LC/MS conditions were optimized, the second part of this study was to evaluate the effectiveness of sample preparation and the impact of sample matrix on the assay. Often when dealing with plasma samples, endogenous matrix such as phospholipids can cause irreproducibility in quantitation due to ionization-suppression effects. Coelution of endogenous matrix with target analytes can result in an arbitrary decrease in the response of target analytes, thus decreasing the overall accuracy of the method3. The determination of matrix interference is a critical aspect in any bioanalytical method development. In this case, a standard protein precipitation method was compared with hybrid zirconia coated silica particles in 96-well SPE format for processing the plasma samples prior to LC/MS analysis.

Experimental

Standard Solutions
Standard solutions were prepared from a stock standard in (3:1) 1% formic acid acetonitrile:water at concentrations of 10, 50, 100, 200 and 300 ng/mL.

Preparation and Extraction of Plasma Samples
Rat plasma stabilized with K2EDTA was acquired from Lampire Biological Laboratories (Pipersville PA). Plasma was spiked directly from stock standard to 400 ng/mL each analyte. Concentration of final sample work up in both techniques is equivalent to 100 ng/mL.

Standard protein precipitation method: Apply 100 µL of spiked plasma to centrifuge vial, followed by 300 µL of 1% formic acid in acetonitrile. Agitate via vortex for two minutes, place vial in centrifuge and spin at 15,000 rpm for 2.5 minutes. Collect supernatant and analyze directly.

HybridSPE®-Phospholipid 96-well method: Apply 100 µL of spiked plasma to the well, followed by 300 µL of 1% formic acid in acetonitrile. Agitate via vortex for four minutes, place on vacuum manifold and apply 10" Hg vacuum for four minutes. Collect filtrate and analyze directly.

LC/MS Analysis of Antiarrhythmic Drugs and Metabolite on Ascentis Express HILIC

Figure 2. LC/MS Analysis of Antiarrhythmic Drugs and Metabolite on Ascentis Express HILIC

Conditions
LC-MS system:
Agilent 1290, 6210 TOF; column: Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm (Product No. 53939-U); mobile phase: (A) 5 mM ammonium formate; (B) 5 mM ammonium formate in acetonitrile, 5:95 (A:B), adjusted to pH 7.0 with formic acid; flow rate: 0.4 mL/min; pressure: 90 bar; column temp.: 35 °C; detection: ESI+, 100-1000 m/z; sample: each compound, 300 ng/mL in 75:25 (1% formic acid acetonitrile:water); injection: 0.5 µL

Results

Figure 2 shows the chromatographic resolution of the antiarrhythmic drugs on the Ascentis Express HILIC. Analysis was conducted on a time of flight (TOF) LC/MS and demonstrated a linear analyte response across the concentration range as shown in Figure 3.

Antiarrhythmic Drugs Calibration Curve

Figure 3. Antiarrhythmic Drugs Calibration Curve

Conditions: same as Figure 2

Using the standard protein precipitation technique, matrix interference was observed with the overlap of endogenous phospholipids and the target analytes. As shown in Figure 4, the elution region of amiodarone, desethylamiodarone and flecainide overlapped with the elution of the endogenous phospholipids. This raised concern for possible detection irregularities. As observed in the matrix trace, phospholipids were carried over from previous sample injections causing real concern for method reproducibility. When analyte levels were calculated against the calibration curve, the concentration of desethylamiodarone and flecainide were substantially lower than the spiked levels. Significant ion suppression was observed due to the endogenous phospholipids in the plasma samples. As shown in Table 1, the coefficient of variation (% C.V.) value was extremely high for both desethylamiodarone and flecainide further confirming the concern of matrix interference.

 

LC-MS Analysis of Antiarrhythmic Drugs in Spiked Plasma following Sample Prep using Protein Precipitation

Figure 4. LC-MS Analysis of Antiarrhythmic Drugs in Spiked Plasma following Sample Prep using Protein Precipitation

Conditions
LC-MS system:
Agilent 1290, 6210 TOF; column: Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm (Product No. 53939-U); mobile phase: (A) 5 mM ammonium formate; (B) 5 mM ammonium formate in acetonitrile, 5:95 (A:B), adjusted to pH 7.0 with formic acid; flow rate: 0.4 mL/min; pressure: 90 bar; column temp.: 35 °C; detection: ESI+, 100-1000 m/z; sample/matrix: rat plasma, unfiltered K2EDTA, spiked with each compound at 100 ng/mL (3:1,1, plasma: 1% formic acid in acetonitrile); injection: 0.5 µL

 

Table 1. Recovery Data: Protein Precipitation Method

Recovery (n=16) Calculated Concentration (ng/mL)
Lidocaine Amiodarone Desethylamiodarone Flecainide
Average 118.5 105.5 52.9 44.7
Std. Dev. 9.6 1.5 21.8 20.5
% C.V. 8.1 1.5 41.2 45.9

 

To validate that the decreased detected levels of desethylamiodarone and flecainide were due to phospholipid ion suppression, plasma samples were processed using the HybridSPE-Phospholipid 96-well plate. This technology utilizes hybrid zirconia coated silica particles to facilitate the selective extraction of phospholipids from biological samples via Lewis acid/base interaction between the phosphate moiety of the phospholipids and the zirconia surface. This interaction is highly selective toward the isolation of phospholipids while remaining non-selective towards target analytes. Sample processing using the HybridSPE-Phospholipid consisted of the addition of formic acid acetonitrile to the plasma samples, followed by mixing and filtration by applying vacuum to the 96-well plate. This technique allowed for direct depletion of sample proteins and phospholipids, resulting in a simplified and effective sample preparation. Samples processed using the HybridSPE-Phospholipid technique were depleted of phospholipid matrix with no additional sample processing.

Figure 5 shows the analyte and phospholipid trace of plasma samples processed using the HybridSPE-Phospholipid technique. Notice the increased peak response of both desethylamiodarone and flecainide as compared to samples processed using the standard protein precipitation technique. The calculated levels were consistent with the spiked levels. There was no phospholipid matrix observed in the HybridSPE-Phospholipid processed samples, confirming the reduced response of desethylamiodarone and flecainide in the standard protein precipitation technique was a result of the endogenous phospholipids. The consistency in the calculated levels of all three antiarrhythmic agents and metabolite with the spiked level coupled with low CV values (Table 2) verifies the precision and accuracy of the HybridSPE-Phospholipid sample preparation.

LC-MS Analysis of Antiarrhythmic Drugs in Spiked Plasma following Sample Prep Using HybridSPE-Phospholipid

Figure 5. LC-MS Analysis of Antiarrhythmic Drugs in Spiked Plasma following Sample Prep Using HybridSPE-Phospholipid

Conditions: same as Figure 4

 

Table 2. Recovery Data: HybridSPE-Phospholipid Method

Recovery (n=16) Calculated Concentration (ng/mL)
Lidocaine Amiodarone Desethylamiodarone Flecainide
Average 108.4 109.8 104.8 119.0
Std. Dev. 5.4 4.4 4.7 7.2
% C.V. 4.9 4.0 4.5 6.1

Conclusions

Endogenous phospholipids can dramatically impact the precision and accuracy of a bioanalytical method. During development, the impact of sample matrix on analyte detection should be a priority in method validation. Failure to do so can result in inaccuracies in reported levels, thus impacting the assessment of patient health. Eliminating matrix effects in LC/MS is imperative to producing reliable and accurate bioanalytical methods. The targeted phospholipid selectivity of the HybridSPE-Phospholipid technique enables simplified sample processing with no phospholipid matrix interference, while exhibiting excellent recovery from plasma and serum samples. This application demonstrates how selectivity in both sample preparation and chromatographic separation allows for a simplified and efficient bioanalytical method resulting in a highly precise and accurate assay. The fast HILIC chromatography on Ascentis Express paired with the selective sample preparation of HybridSPE-Phospholipid, LC-MS Ultra solvents and Cerilliant® Certified Reference Materials resulted in a robust and precise bioanalytical method.

Legal Information

Ascentis, and HybridSPE are registered trademarks of Sigma-Aldrich Co. LLC
Cerilliant is a registered trademark of Cerilliant Corporation

Materials

     

 References

  1. American Heart Association, Medications for Arrhythmia. http://www.heart.org (accessed Nov. 26, 2012).
  2. Ascentis Express HILIC Guide. Faster Analysis of Polar Compounds. 2012. Sigma-Aldrich Literature Code OMI.
  3. Aurand, C. Understanding, Visualizing, and Reducing the Impact of Phospholipid- Induced Ion Suppression in LC-MS. Supelco Reporter, 2012, 30.2, 10-12.

 

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