SPME-Overcoated Fiber: Physically Robust and Resistant to Fouling

High background samples, such as many foods, pose a challenge to immersion SPME due to the presence of fats, sugars, pigments, and other macromolecules. These can stick to the SPME fiber and reduce its usable life and/or be transferred to the GC, where they may interfere with chromatographic analysis. A new development in SPME fiber technology incorporates a protective overcoating on the fiber. This overcoating, which consists of polydimethylsiloxane (PDMS) protects the fiber, making it more physically robust and less prone to chemical fouling.

 

Overcoated SPME Fiber (PDMS/DVB)

Overcoated SPME Fiber (PDMS/DVB)

 

Appearance of Overcoated  and Non-Overcoated PDMS/DVB SPME Fibers After Repeated Immersion Extractions of Spaghetti Sauce

Appearance of Overcoated and Non-Overcoated PDMS/DVB SPME Fibers
After Repeated Immersion Extractions of Spaghetti Sauce

 

Pesticide Response from Spiked Spaghetti Sauce Extracted by Immersion SPME; Overcoated and Non-Overcoated PDMS/DVB Fibers. Using the Non-Overcoated (non OC) Fiber, a Complete Loss in Response Occurred After 19 Extractions Due to GC System Contamination

Pesticide Response from Spiked Spaghetti Sauce Extracted by Immersion SPME; Overcoated
and Non-Overcoated PDMS/DVB Fibers. Using the Non-Overcoated (non OC) Fiber, a Complete
Loss in Response Occurred After 19 Extractions Due to GC System Contamination

 

Materials