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Ferrioxamine E

By: Jvo Siegrist, AnalytiX Volume 5 Article 5

Ferrioxamine E
Scientifically proven supplement for culture media improves detection of Salmonella, Enterobacter sakazakii and Yersinia enterocolitica

By Jvo Siegrist, Product Manager, isiegris@sial.com

Picture 1.  Salmonella detected in a selective enrichment broth (Selenite Broth); left: Salmonella, right: control

 

Ferrioxamine E from Streptomyces antibioticum is a siderophore which facilitates the supply of iron (III), an essential trace element, to bacteria involved in food poisoning, including Salmonella, Enterobacter sakazakii and Yersinia enterocolitica. It promotes rapid growth by reducing the lag phase in culture media and reactivates dormant bacteria. Use of ferrioxamine E can permit rapid detection of low bacterial cell counts, even from dry products like spices and tea after long storage periods. It is an essential component of quality control efforts in the food industry.

Ferrioxamine E is often used in Buffered Peptone Water, the medium recommended by the ISO-Norms for Enterobacteriacea. Ferrioxamine E also improves the motility of Salmonella, which helps to improve the identification by semisolid selective motility media like SIM, MRSV, DIASSALM or SMS. Ferrioxamine E does not improve growth of Escherichia coli, Shigella, Proteus, Providencia and Morganella species; a feature that makes it a semi-selective compound. The recommended concentration of Ferrioxamine E to promote bacterial growth is between 5 and 200 ng/mL.

Part No. Description Package Size
38266 Ferrioxamine E
(1,12,23-Trihydroxy-1,6,12,17,23,28-hexaazacyclotritriacontane-2,5,13,16,24,27-hexone Iron(III) complex)
Usage: Supplementation of pre-enrichment broth and enrichment broth media for improved recovery of Salmonella from artificially or naturally contaminated foods
3 mg, 9 mg

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Materials

     

References

  1. R. Reissbrodt, H. Heier, H. Tschape, R.A. Kingsley, P.H. Williams, Resuscitation by ferrioxamine E of stressed Salmonella enterica serovar typhimurium from soil and water microcosms., Appl. Environ. Microbiol., 66(9), 4128-30 (2000)
  2. R. Reissbrodt, E. Vielitz, E. Kormann, W. Rabsch, H. Kuhn, Ferrioxamine E-supplemented pre-enrichment and enrichment media improve various isolation methods for Salmonella., Int. J. Food Microbiol., 29(1), 81-91(1996)
  3. P. Pless, R. Reissbrodt, Improvement of Salmonella detection on motility enrichment media by ferrioxamine E-supplementation of pre-enrichment culture., Int. J. Food Microbiol., 27, 147-159 (1995)

 

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