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Kit for the determination of hydrocarbon oil index according to DIN EN ISO 9377-2/2000 (H53)

By: Rainer Walz, AnalytiX Volume 4 Article 3

This kit contains not only the dual-layer Florisil®/Na2SO4 SPE tube, but also all the reagents and standards employed in “water quality-determination of hydrocarbon oil index” (DIN EN ISO 9377-2/2000).

By Rainer Walz 

ISO 9377 specifies a method for the determination of the hydrocarbon oil index in water by means of gas chromatography. It is suitable for surface water, waste water and water from sewage treatment plants and allows the determination of hydrocarbon oil index in concentrations above 0.1 mg/L.

The water sample is extracted with a hydrocarbon solvent. Polar substances are removed by cleanup on Florisil®. The purified aliquot is analyzed by capillary chromatography using a non-polar column and a flame ionization detector. The total peak area between n-de-cane and n-tetracontane is measured. The concentration of mineral oil is quantified against an external standard consisting of two specified mineral oils, and the hydrocarbon oil index is calculated [Method principle extracted from the European Standard DIN EN ISO 9377-2/2000. To request a copy of “water quality-determination of hydrocarbon oil index” (DIN EN ISO 9377-2 /2000), please contact the DIN institute in Berlin, Germany, www2.din.de].

 


Mineral oil sample after Florisil® absorption ......... 
GC chromatogram done as recommended by DIN EN ISO 9377-2/2000 (H53)
Column: Supelco HT-5 6 m x 0.53 mm I.D., fused silica, Df 0.1 μm (Cat. No. 25004)
Injection temp.: 40 °C to 300 °C
Injection volume: 2 μL
Carrier gas: helium
Temp. program: 40 °C/5 min., 10 °C/min. to 300 °C, 300 °C/5 min.
Detector: FID

 

Inside the kit, you can find the new dual layer glass Florisil®/Na2SO4 SPE Tube. These tubes have Na2SO4 in the upper layer and Florisil® (magnesium silicate) in the lower layer, separated and packed with PTFE frits. The upper Na2SO4 layer aids in removing aqueous sample residues that may hinder Florisil® performance and/or subsequent GC analysis.

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