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Collagen Usage Recommendations

By: George Sitterley, BioFiles 2008, 3.8, 4.

Collagen is one of the most abundant proteins in the connective tissues and internal organs of mammals. It provides the tensile strength of the extracellular matrix (ECM). It is classified into a number of structurally and genetically distinct types. Although different types of collagen exist, they are all composed of molecules containing three polypeptide chains arranged in a triple helical conformation. Slight differences in the primary structure (amino acid sequence) establish differences between the types. The amino acid sequence of the primary structure is primarily a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.1,2

Collagen is composed of three polypeptide chains arranged in a triple helical conformation.

Collagen is also used in the study of growth, differentiation, migration of cell lines, and tissue morphogenesis during development.

Collagens

Name Source Storage Temp Target Cells For Attachment Concentration For Use Cat. No.
Collagen, solution from calf skin 2-8°C Muscle cells, hepatocytes, spinal ganglion, embryonic lung cells, Schwann cells. Mediates the attachment of many cell types. Recommended for use as a cell culture substratum at 6-10 μg/cm2. Not suitable for 3D gel formation. 6 ‑ 10 μg/cm2 C8919-20ML
Collagen, powder from chicken sternal cartilage 2-8°C Recommeded for use as a cell culture substratum at 6-10 μg/cm2. 6 ‑ 10 μg/cm2 C9301-5MG
C9301-25MG
C9301-100MG
Collagen, lyophilized powder (from sterilefiltered solution) from Engelbreth- Holm-Swarm murine sarcoma basement membrane −20°C Epithelial cells, endothelial cells, muscle cells, nerve cells Recommended for use as a cell culture substratum at 6-10 μg/cm2. 6 ‑ 10 μg/cm2 C0543-1VL
Collagen, powder from rat tail 2-8°C Muscle cells, hepatocytes, spinal ganglion, embryonic lung cells, Schwann cells. Mediates the attachment of many cell types. Recommended for use as a cell culture substratum at 6-10 μg/cm2. This product may not be suitable for 3-D gel formation. 6 ‑ 10 μg/cm2 C7661-5MG
C7661-10MG
C7661-25MG
C7661-50MG
C7661-100MG
Collagen, powder from kangaroo tail 2-8°C Muscle cells, hepatocytes, spinal ganglion, embryonic lung cells, Schwann cells. Mediates the attachment of many cell types. 6 ‑ 10 μg/cm2 C1809-10MG
Collagen, powder from calf skin 2-8°C Muscle cells, hepatocytes, spinal ganglion, embryonic lung cells, Schwann cells. Mediates the attachment of many cell types. Recommended for use as a cell culture substratum at 6-10 μg/cm2. 6 ‑ 10 μg/cm2 C9791-10MG
C9791-50MG
C9791-100MG
C9791-250MG
Collagen, powder from human placenta −20°C Epithelial cells, endothelial cells, muscle cells, nerve cells 6 ‑ 10 μg/cm2 C5533-5MG

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Materials

     

References

  1. Tanzer, M. L., Cross-linking of collagen. Science, 180(86), 561-566 (1973).
  2. Bornstein, P., and Sage, H., Ann. Rev. Biochem., 49, 959 (1980).

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