CompoZr® Zinc Finger Nucleases

By: Savita Bagga, PhD., BioFiles v7 n3, 2012, 19

BioFiles Volume 7, Number 3 — Epigenetics

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Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations.

Double-strand breaks are important for sitespecific mutagenesis in that they stimulate the cell's natural DNA repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ). By implementing established, field-proven methods, these processes are harnessed to generate precisely targeted genomic edits, resulting in cell lines with targeted gene deletions, integrations, or modifications.

CompoZr ZFNs are used to create modified cell lines with targeted gene deletions, gene insertions, or gene corrections that can be used in studying epigenetics.

Benefits

  • Rapid disruption of, or integration into, any genomic locus
  • Mutations made are permanent and heritable
  • Works in a variety of mammalian somatic cell types
  • Edits induced through a single transfection experiment
  • Knockout or knockin cell lines in as little as two months
  • Single or biallelic edits occur in 1–20% of clone population
  • No antibiotic selection required for screening
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Materials

     
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