Organelle Isolation

By: Mark Frei, BioFiles v6 n5, 22–25

BioFiles Volume 6, Number 5 — Centrifugation

Download BioFiles v6 n5 (4.66 Mb PDF)

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples. To be suitable for further analysis, isolated organelles must be relatively pure, and structurally and functionally intact. It is also important to verify the organelle integrity with robust assays.

Sigma® Life Science offers several organelle isolation and enrichment kits, companion organelle functionality assays, and antibodies that are suitable for evaluation of samples from plant, animal, and cell culture sources.

Antibodies
Application Abbreviation Table
Materials

Antibodies

Product Name Host Clone No. Form Gene Symbol Species Reactivity Application Cat. No.
Anti-GM130 (C-terminal) rabbit affinity isolated antibody Golga2, rat
Golga2, mouse
GOLGA2, human
human
mouse
rat
IF (i)
IP
WB CL
G7295-200UL
Anti-GRP78/BiP (ET-21) rabbit IgG fraction of antiserum Hspa5, mouse
HSPA5, human
Hspa5, rat
Xenopus
chicken
hamster
human
mouse
rat
ICC
WB
G9043-200UL
Anti-Nuclear Pore Complex
Proteins
mouse 414 purified immunoglobulin Xenopus
human
mouse
rat
yeast
EM
ICC
IP
WB
N8786-200UL
Anti-PMP70 rabbit affinity isolated antibody Abcd3, mouse
ABCD3, human
Abcd3, rat
human
mouse
rat
IF (i)
IP
WB CL
P0497-200UL

Application Abbreviation Table

Application Abbreviation   Application Abbreviation
ANA-indirect immunofluorescence IF (ANA) Immunohistochemistry IHC
Capture ELISA ELISA (c) Immunohistochemistry (formalin-fixed, paraffin-embedded sections) IHC (p)
Direct ELISA ELISA (d)
Direct immunofluorescence IF (d) Immunohistochemistry (frozen sections) IHC (f)
Dot blot DB Immunoprecipitation IP
Dot immunobinding DIBA Indirect ELISA ELISA (i)
Electron microscopy EM Indirect immunofluorescence IF (i)
Enzyme immunoassay EIA Microarray ARR
Flow cytometry FACS Neutralization Neutral
Immunoblotting WB Ouchterlony double diffusion ODD
Immunoblotting (chemiluminescent) WB CL Particle immunofluorescence PIFA
Immunocytochemistry ICC Quantitative precipitin assay QPA
Immunoelectrophoresis IEP Radioimmunoassay RIA

Materials

     
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