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Vitronectin Cell Attachment Protocol

By: George Sitterley, BioFiles 2008, 3.8, 13.

BioFiles 2008, 3.8, 13.

Vitronectin (Cat. Nos. V8379, V0132, V9881)

Optimal conditions must be determined for each cell line and application.

  1. Reconstitute with tissue culture grade water and sterilize by filtration.
  2. Material is reported to be active at a concentration of 0.1 mg/cm2 of surface area. Optimal concentrations vary with each cell line.
  3. Coat culture surface for 1-2 hours at 37°C. Remove any remaining solution and wash with a balanced salt solution before introducing cells and medium.

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