Bløk® Noise Cancelling Reagents
- Protein-free for decreased background and enhanced detection
- Eliminates the need to run a second gel for Coomassie staining.
- Stable at room temperature for 1 year
Bløk®-FL reagent provides exceptional signal-to-noise results. An Immobilon®-FL blots with dilution series of EGF-stimulated A431 lysate were blocked with indicated blocking reagent and incubated with anti-GAPDH antibody 1:10,000, (Catalog No. MAB374) diluted in the blocker. Following probing with secondary anti-mouse IgG antibody IRDye680), the blot was scanned on the Odyssey® scanner (LI-COR) following one hour of vacuum drying.
| Product No. |
Description |
Add to Cart |
|---|
| WBAVDCH01 |
Bløk®-CH Reagent Chemiluminescence detection 500 mL/bottle |
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| WBAVDFL01 |
Bløk®-FL Reagent Fluorescence detection 500 mL/bottle |
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| WBAVDP001 |
Bløk®-PO Reagent Phosphoprotein detection 500 mL/bottle |
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New Immobilon® Signal Enhancer combines signal amplification and blocking in one ready-to-use reagent.
Boost signal: Amplify low signal intensity Western blots—without amplifying noise
Save primary antibody: Use Immobilon® Signal Enhancer to reduce the amount of valuable primary antibody required
| Product No. |
Description |
Add to Cart |
|---|
| WBSH0500 |
Immobilon® Signal Enhancer for Immunodetection |
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Chemiluminescent Signal Amplification
Fluorescent Signal Amplification
Two-fold dilution series of EGF-stimulated A431 cell lysate were resolved by SDS-PAGE and transferred onto Immobilon®-P or Immobilon®-FL membrane. Blots were blocked with either 5% non-fat dry milk or Immobilon® Signal Enhancer. Primary antibodies (rabbit anti-Akt1, 05-796 and mouse anti-PKC antibody, 05-983) and the labeled secondary antibodies were diluted in the respective reagents at identical dilution ratios. All blots were compared under the same conditions.
