WellRED Primers

Sigma is licensed by Beckman Coulter, Inc., to supply WellRED dye-labeled oligos designed for use with the CEQ/GeXP Genetic Analysis Systems and may be used for direct hybridization or in PCR amplification. WellRED dye-labeled oligos are DNA oligonucleotides labeled with WellRED dyes, D2, D3 or D4, at the 5'end.

WellRED Specifications

Yields Cartridge: 2 and 4 OD
HPLC: 5, 10, 50 and 100 OD
PAGE: 1 OD
Purification Cartridge, HPLC
Length 15 – 30 bases (inquire for longer lengths)
Backbone Phosphodiester
Format Supplied Dry
Modifications D2-PA, D3-PA or D4-PA at the 5’end
Packaging 2 mL Opaque Tubes
Quality Controls MALDI-TOF MS, Electrospray Ionization MS, OD by UV Spectroscopy
Technical Datasheet & Labels Includes yield, Tm (melting temperature), MW, and µg/OD
Duplicate set of labels with each order
Additional Services Aliquoting into tubes
MSQC traces available upon request
Shipping Schedule Shipped within 4 to 5 business days

Benefits of WellRED Dye Chemistry

  • WellRED oligos use cyanine-based fluorescent dyes with high extinction coefficients that absorb in the near-infrared region, thereby reducing background noise from biological materials, resulting in highly sensitive detection.
  • The CEQ/GeXP Genetic Analysis Systems enable DNA samples to be kept in linear form during analysis. The CEQ/GeXP Genetic Analysis Systems' online denaturation process prevents any reformation of secondary structures, especially during a long run without any manual steps.
  • WellRED dye chemistry and pre-heating enable the CEQ/GeXP Genetic Analysis Systems to resolve complex structures, where other systems fail.

Yields and Estimated Number of Assays

Yield (OD) 2 4 5 10
Purification Cartridge Cartridge HPLC HPLC
Approx. yield (nmols*) 10 20 25 50
Estimated assays (reactions) 1,000-2,000 2,000-4,000 2,500-5,000 5,000-10,000

*Estimate 1 OD = 5 nmols = 30 µg, for a 20 base oligo

Spectral Properties of WellRED Dyes

WellRED Oligo Excitation maximum (nm) Emission maximum (nm) Molar extinction coefficient
D2-PA 750 770 170,000
D3-PA 685 706 224,000
D4-PA 650 670 203,000

CEQ/GeXP is a trademark of Beckman Coulter, Inc.

Materials