Duolink® References

Duolink proximity ligation assay (PLA) has been used in countless studies in a variety of fields. Browse the following tables to view highlights from the many applications of this technology.

Cancer
Neuroscience
Cell Biology
Epigenetics
Immunology

The abbreviations used on this page are as follows:

Application

  • PPI: Protein-protein interaction
  • PTM: Post-translational modification
  • SP: Single protein

Detection

  • BF: Brightfield
  • FL: Fluorescence
Cancer
Title Application Detection Sample Reference
CYLD negatively regulates transforming growth factor-β-signalling via deubiquitinating Akt. PPI FL Cell Lim JH et al. Nat. Commun. 2012 Apr 10; 3: 771.
SLUG is required for SOX9 stabilization and functions to promote cancer stem cells and metastasis in human lung carcinoma. PPI FL Cell Luanpitpong S et al.
Oncogene. 2016 Jun 2;
35 (22): 2824-33.
Angiopoietin-like 4 protein elevates the prosurvival intracellular O2(-):H2O2 ratio and confers anoikis resistance to tumors. PPI / PTM FL Cell / Tissue Zhu P et al. Cancer Cell. 2011 Mar 8; 19 (3): 401-15.
Membranous expression of ectodomain isoforms of the epidermal growth factor receptor predicts outcome after chemoradiotherapy of lymph node-negative cervical cancer. PTM BF Tissue Halle C et al. Clin Cancer Res. 2011 Aug 15; 17 (16): 5501-12. 
Monosomy of chromosome 10 associated with dysregulation of epidermal growth factor signaling in glioblastomas. SP FL Tissue Yadav AK et al. JAMA. 2009 Jul 15; 302 (3): 276-89. 


 
Neuroscience
Title Application Detection Sample Reference
Necdin protects embryonic motoneurons from programmed cell death. PPI FL Tissue Aebischer J et al. PLoS One. 2011; 6 (9): e23764.
Golden Goal collaborates with Flamingo in conferring synaptic-layer specificity in the visual system. PPI FL Cell Hakeda-Suzuki S et al. Nat. Neurosci. 2011 Mar; 14 (3): 314-23.
Local translation of extranuclear lamin B promotes axon maintenance. PPI FL Cell Yoon BC et al. Cell. 2012 Feb 17; 148 (4): 752-64
Endothelin-converting enzyme-1 regulates trafficking and signalling of the neurokinin 1 receptor in endosomes of myenteric neurones. PPI / PTM FL Cell Pelayo JC et al. J Physiol. 2011 Nov 1; 589 (Pt 21): 5213-30.
AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo. SP FL Cell Domise M et al. Sci Rep. 2016 May 27; 6: 26758
 
Cell Biology
Title Application Detection Sample Reference
LRP2 is an auxiliary SHH receptor required to condition the forebrain ventral midline for inductive signals. PPI BF Tissue Christ A et al. Dev Cell. 2012 Feb 14; 22 (2): 268-78.
Notch-independent RBPJ controls angiogensis in the adult heart. PPI FL Cell Diaz-Trelles R et al.  Nat Commun. 2016 Jun 30; 7: 12088.
The polarisome is required for segregation and retrograde transport of protein aggregates. PPI FL Cell Liu B et al. Cell. 2010 Jan 22; 140 (2): 257-67.
Tissue factor and PAR1 promote microbiota-induced intestinal vascular remodelling. PPI FL Cell / Tissue Reinhardt C et al. Nature. 2012 Mar 11; 483 (7391) :627-31.
The p53 mRNA-Mdm2 interaction controls Mdm2 nuclear trafficking and is required for p53 activation following DNA damage. PPI / PTM FL Cell Gajjar M et al. Cancer Cell. 2012 Jan 17; 21 (1): 25-35.
Tobacco calmodulin-like protein provides secondary defense by binding to and directing degradation of virus RNA silencing suppressors. PPI / SP FL Cell Nakahara KS et al. Proc Natl Acad Sci USA. 2012 Jun 19; 109 (25): 10113-8. 
 
Epigenetics
Title Application Detection Sample Reference
PTPRO represses ERBB2-driven breast oncogenesis by dephosphorylation and endosomal internalization of ERBB2. PPI FL Cell Dong H et al. Oncogene. 2017 Jan 19; 36 (3): 410-422.
Dnmt3/transcription factor interactions as crucial players in targeted DNA methylation. PPI / PTM FL Cell Hervouet E et al.  Epigenetics. 2009 Oct 21; 4 (7): 487-99.
Methyl donor deficiency impairs fatty acid oxidation through PGC-1α hypomethylation and decreased ER-α, ERR-α, and HNF-4α in the rat liver. PPI / PTM FL Tissue Pooya S et al.  J Hepatol. 2012 Aug; 57 (2): 344-51.
Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA. PPI / PTM / SP FL Cell Yu Y et al. Mol Cell. 2012 Apr 13; 46 (1): 7-17.
KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis. SP FL Cell / Tissue Shankman LS et al.Nat Med. 2015 Jun; 21 (6): 628-37
 
Immunology
Title Application Detection Sample Reference
Macrophage receptor SR-AI is crucial to maintain normal plasma levels of coagulation factor X. PPI FL Cell Muczynski V et al. Blood. 2016 Feb 11; 127 (6): 778-86.
Toll-like receptor 2 mediates the activation of human monocytes and endothelial cells by antiphospholipid antibodies. PPI FL Cell Satta N et al. Blood. 2011 May 19; 117 (20): 5523-31.
NF-κB-mediated degradation of the coactivator RIP140 regulates inflammatory responses and contributes to endotoxin tolerance. PPI / PTM FL Cell Ho PC et al.  Nat Immunol. 2012 Mar 4; 13 (4): 379-86.
Histone deacetylases 6 and 9 and sirtuin-1 control Foxp3+ regulatory T cell function through shared and isoform-specific mechanisms. PTM FL Cell Beier UH et al. Sci Signal. 2012 Jun 19; 5 (229): ra45.
Interleukin-10 genotypes of the -1087 single nucleotide polymorphism influence sp1 expression in periodontitis lesions. SP BF Tissue Larsson L et al. J Periodontol. 2011 Sep; 82 (9): 1376-82.

Materials