Muse® Cell Analyzer

Get quantitative, three-parameter data from this user-friendly, benchtop device, so you can make critical cell health measurements as soon as you need them.

Applications of the Muse Cell Analyzer

  • Cell viability
  • Apoptosis
  • Cell cycle progression
  • DNA damage response
  • Cell cycle effects
  • Signaling pathway activation

Why? Because benchtop cell health analysis can directly affect your chances of experimental success.

Simple
cell-by-cell analysis, now at your side.

A lot can happen during a cell's journey from healthy function to cell death. Knowing that cells die is not enough—determining how they die is critical to the development of safe pharmaceuticals, and to ensuring that results from cultured cells reflect the systems they are intended to model.

Advantages of the Muse Cell Analyzer

Built on flow cytometry principles, the Muse system uses microcapillary fluidics and pre-optimized reagents to create a compact system that requires little setup and no expertise. Together with a touchscreen interface and intuitive software, these attributes make the system a faster, easier alternative to more time-consuming methods like Western blot (that may also demand considerable technical expertise) for routine analysis of cell culture health, and to assess the effects of compounds for toxicology and drug discovery screening.

Instrument Characteristics and Dimensions
Cell Number per Assay User defined; cell concentrations of 10,000–500,000 cells/mL
Sample Format Single loader
Sample Volume 200 µL
Emission max. Yellow: 28nm bandwidth at 576nm
Red: 30nm bandwidth at 680nm
Forward scatter = laser wavelength
Excitation max. Green Laser (532nm)
Scatter Forward
Throughput Less than 2 minutes per sample
Dimensions height: 8.69 inches (220.75 mm)
width: 8.12 inches (206.37 mm)
depth: 11.11 inches (282.15 mm)
Output Data File Formats • Binary data storage in Flow Cytometry Standard (FCS) 3.0 format
• Spreadsheet results file in comma-separated value (CSV) format


Workflow comparison among protein detection methods.

For proteins not really in solution, bulk methods require lysis of samples to render proteins accessible to detection. Reagents, supplies and and equipment for separation, transfer, probing, detection and imaging may be needed, depending on technique. Lengthy primary antibody incubations and serial washes following each binding step can result in significant hands-on and total elapsed time for routine protocols. The Muse Cell Analyzer uses preoptimized reagent cocktails to minimize variation and instrument setup, resulting in appreciable reductions in time spent on the bench and total start-to-result time.
 

Assay Key Materials and Equipment Needed Hands-on Time Typical Total Assay Time:
From Cells to Data
ELISA Coated multiwell plates
Capture antibody
Detection antibody conjugate
Enzyme substrate
Stop solution
Plate washer
ELISA reader
1.5 hours 8 hours – 2.5 days
Dot blot Blot cassette
Blocking buffer
Nitrocellulose membrane
Protein standard
Primary antibody
Secondary antibody
Detection reagent
1.25 hours 5.5 hours
Traditional Western
blot protocols
Lysis buffer
Protein quantification kit
Protein standards
SDS page gels
Electrophoresis chamber
Loading buffer
Running buffer
Transfer buffer
Protein transfer chamber
Membranes
Filter paper
Blocking buffer
Primary antibody
Secondary antibody
Gel/blot imager
2 – 3 hours 10 hours – 2.5 days*
Muse Cell Analyzer Muse Cell Analyzer
Relevant Muse assay
10 – 15 minutes 10 minutes – 4 hours

*Significant time savings can be achieved by use of a vacuum-driven method such as the SNAP i.d.® 2.0 system for Western blotting.




 

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